The advanced version of CHRONECT Symbiosis enables powerful UHPLC up to 1000 bar.

This system offers all the advantages of online SPE and simultaneous SPE sample preparation and LC-MS analysis. Additionally, the Online-SPE system is fully compatible with UHPLC columns up to 1000 bar using disposable PEEK-SPE cartridges with 10 x 1 mm ID. Due to the reduced SPE cartridge volumes (8 µL, 10 x 1 mm ID) this system is compatible with the lower UHPLC flow rates without losing UHPLC resolution. Multi-component UHPLC-MS analysis including fully automated sample preparation within 2-4 minutes can be achieved.

The system offers all the advantages of online SPE to increase assay performance while reducing method development time and manual sample preparation steps. With the Solvent Selector in the SPH1299 pumps and the optional Column Selector in the new Mistral™ you can also run multiple batches under different LC conditions. You can even choose to perform direct injection or extraction without reconfiguring your system hardware. The mixing and dilution routine is handled by the innovative and flexible CHRONECT Robotic Autosampler. Sample preparation steps like the addition of internal standard can also be performed.

While the cleaning is performed on the left cassette, the SPE-UHPLC-MS can run simultaneously on the right cassette. Forward and backward washing is possible, providing clean samples and high assay sensitivity when performing your multi-component UHPLC analysis. Peak focusing can be realized with mixed SPE cartridges and an additional SPH1299 UHPLC gradient pump.

CHRONECT Symbiosis is controlled by CHRONOS Symbiosis. CHRONOS controls the entire system in an efficient and intelligent way and increases the sample throughput by overlapping processes. The cartridge is prepared while the analysis is running. CHRONOS organizes the connection to the LC-MS. All CHRONECT Symbiosis systems process multiple batches quickly and reliably with highest analytical performance, completely unattended.